WebThe ideal point spread function ( PSF) is the three-dimensional diffraction pattern of light emitted from an infinitely small point source in the specimen and transmitted to the image plane through a high numerical aperture ( NA) objective. It is considered to be the fundamental unit of an image in theoretical models of image formation. WebOct 17, 2024 · Therefore, high NA objectives often have short working distances, which makes it impossible to image an entire non-sectioned brain sample, because the sample …
Reflective multi-immersion microscope objectives inspired by the ...
WebJul 28, 2024 · An objective lens with a low numerical aperture (NA) has a large field of view but poor resolution. In contrast, a high NA objective lens will have a higher resolution but reduced field of view. In an effort to close the gap between these trade-offs, we introduce an acoustofluidic scanning nanoscope (AS-nanoscope) that can simultaneously ... date ideas tacoma wa
arXiv.org e-Print archive
WebField of view and scanning regimes A single exposure of our camera captures a (tilted) 2D FOV. Modern sCMOS chips are large enough that our 2D FOV is typically limited by the … WebThis lens has the industry’s highest NA for a 60X water immersion objective lens, maintaining flatness throughout the field of view while showing good correction of chromatic aberration over a wide range of wavelengths. Numerical aperture: 1.27 Super image flatness Chromatic aberration correction: from visible to infrared. WebWhere d(tot) represents the depth of field, λ is the wavelength of illuminating light, n is the refractive index of the medium (usually air (1.000) or immersion oil (1.515)) between the coverslip and the objective front lens element, and NA equals the objective numerical aperture. The variable e is the smallest distance that can be resolved by a detector that is … date ideas south florida