Phis-sumo vector
Webb11 jan. 2016 · Hexahistidine small ubiquitin-like modifier (SUMO) tagged constructs were generated by cloning the above sequences into a pHis-SUMO vector (Senturia et al. … WebbIncludes: The Champion pET SUMO TA Cloning box contains linearized Champion pET SUMO vector, sterile water, dNTPs, 10X PCR Buffer, control template and primers, T4 DNA ligase, 10X ligation buffer, primers for sequencing or PCR screening, and an expression control. The SUMO Protease box contains SUMO Protease and buffers.
Phis-sumo vector
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Webb1 dec. 2009 · The entire resulting cDNAs were sequenced to confirm the site-directed mutations. pFLAG-Sumo-2 and pHis-Sumo-2 cDNAs were constructed by inserting the … http://www.biofeng.com/zaiti/dachang/pE-SUMO.html
WebbExamples of UBLs are SUMO, Rub1 (also called Nedd8), ISG15, Apg8 and Apg12. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA … Webb7 juli 2014 · Hexahistidine small ubiquitin-like modifier (SUMO) tagged constructs were generated by cloning the ANKS3-SAM and ANKS6-SAM sequences into a pHis-SUMO …
Webb1. Centrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g. 5.Store the plasmid at -20 ℃. http://www.biofeng.com/zaiti/dachang/pSUMO.html
Webb中文名称： His和SUMO双标签质粒 英文名称： pET-N-His-PreScission-SUMO 产品规格： 1μg 100μg 发货周期： 1～3天 产品价格： 询价 本产品仅可用于科研实验，严禁用于其他 …
WebbpET SUMO (linearized) Linearized bacterial vector with 3'-T overhangs for TA cloning and inducible expression of an N-terminally 6xHis- and SUMO-tagged protein. Sequence … how did alexander bell invent the telephoneWebbvariable. A vector xi 2Rm can be assigned one of four disjoint point sets y1, y2, y3 or y4 within an m-dimensional feature space. xi 2Rm is the ith training data row and yi … how did alexa demie start actingWebbSUMO-4V constructs were made to express active recombinant proteins ending in the terminal di-glycine residues (Fig. 1, additional C-terminal residues are cleaved normally in … how did alexander corvinus become immortalWebbThe IsdH expression vector used in this study was a modi- fied version of the pRM208 plasmid, which contained the second and third NEAT domains and the intervening … how many runways does o\u0027hare airport haveWebb26 maj 2024 · 载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically … how many rupees in one dollarWebbCentrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. … how did alexander become ruler of egyptWebb11 dec. 2016 · pSUMO大肠杆菌表达载体说明 how many rupees to a pound