Rpl22ha
WebDec 17, 2024 · The transcriptome of enteric glial cells (EGCs) was investigated using PLPCreERT2 Rpl22HA mice. In addition, we assessed the effect of supernatant from neurosphere-derived EGCs on monocyte differentiation based on the expression of pro- and anti-inflammatory factors. Results: Muscularis inflammation induced marked alterations … WebRibosomal protein L22 ( Rpl22 Mouse) Use Tags HA Expression Mutation Promoter Availability Academic Institutions and Nonprofits only Enlarge pVP91A-pcaHG Plasmid #113766 Purpose Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida. Depositor John Lipscomb …
Rpl22ha
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Web2009. Cell-type-specific isolation of ribosome-associated mRNA from complex tissues. Sanz E , et al. PubMed: 19666516. MGI: J:151962. Proc Natl Acad Sci U S A 106 (33):13939-44. … WebSep 28, 2024 · (A) Tie2-Cre; Rpl22HA/HA ( Ribo-Tag) mice, which are designed for expressing HA-tagged ribosomal protein in BECs. (B) Immunohistochemistry for Ai9 (Cre …
WebOct 10, 2024 · A protocol to isolate highly pure mRNAs from BECs in the cortex of eight- to twelve-week-old male Tie2-Cre; Rpl22HA/HA mice is established by using a cell strainer to trap blood vessels prior to immunoprecipitation and according to the results of RT–PCR, the specificity of the mRNA pools isolated by the protocol was much higher than that of the … WebC, Heatmap and relative log2 fold change of significantly altered translated transcripts in the chemokine pathway from neurons (Syn1Cre+/−Rpl22HA/+) and astrocytes (GFAPCre+/−Rpl22HA/+) in the OB of infected mice (each column represents one individual mouse, adjusted p 0.05). D, Infected mice were perfused at 6 dpi and brains were …
WebSpecifically, we used Cx 3 cr1 creER mice 26 in combination with a Rpl22 HA RiboTag allele 27 , in which tamoxifen (TAM)-induced Cre recombinase activation leads to permanent expression of... WebUpon Cre-mediated recombination of their gene-targeted locus, Rpl22HA/+ mice express an HA-tagged ribosomal protein L22, enabling the immunoprecipitation of ribosome-bound RNA. We inoculated neonatal Rpl22HA/+ mice with rLCMV-Cre, inducing HA-tagged ribosomal expression in infected neurons. Mice were sacrificed 9 days after challenge …
WebApr 1, 2024 · Standard lead time ex-works. 1 Day/Days. Net Weight (kg) 0,065 Kg. Packaging Dimension. 45,00 x 51,00 x 64,00. Package size unit of measure. MM. Quantity Unit.
WebApr 24, 2024 · Using mice expressing an exogenous HA tag on ribosomes (Rpl22HA) specifically in astrocytes ( Aldh1l1 -cre/ERT2 x Ribotag mice) we sequenced polysomic … prony resources stock priceWebXhoI XbaI pZac2.1_gfaABC1D_Rpl22HA 4955 bp. Created Date: 5/8/2024 10:54:54 PM lace weave closureWebNational Center for Biotechnology Information prony shear relaxation ansysWebWe used standard methods ( Figure 7 C,D) to immunoprecipitate (IP) Rpl22HA containing ribosomes and their associated actively translated mRNAs ( Sanz et al., 2009), and … prony resources new caledonia stockWebRiboTag analysis of retinal ECs during postnatal development. a Diagram depicting retinal EC-specific RiboTag transcriptome analysis. Inducible (Tmx controlled) recombination of the RiboTag allele... prony shearWebOct 21, 2024 · In applying this RiboTag strategy for MPs, we retrieved the microglia/macrophage-specific translatomes from Cx3cr1 CreER:Rpl22HA mice . This approach carries important advantages for microglia, including the exclusion of exogenous RNAs from microglial phagocytosis of surrounding cellular elements, allowing … prony series fitWebTranslatome analysis using RiboTag mice, in which Rpl22, a ribosomal component, is tagged by the hemagglutinin epitope under Cre recombinase activation, could serve as an excellent tool that overcomes these caveats. prony resources new caledonia logo